Manual

⇒ Search napRNA of genome

• Step1: Users can select a target genome on the homepage by clicking the “Homo sapiens” module or “Mus musculus” module, then choose a napRNA type, and click “Submit” to view information about this type of napRNA in the genome.
• Step2: Click "Browser" to display the detail information about this napRNA. And the details will be displayed in the detail information section that is below the query page.

⇒ Evolution

• Step1: Users can click the "Evolution" module on the homepage to search for napRNA in other species.
• Step2: Select a clade of interest, then choose your target organism, and finally select the napRNA type before clicking "Submit". Information about this napRNA in the genome will be displayed on a new page.
• Step3: Click "napRNA ID" to display the detail information about this napRNA. Click "Human ID" or “Mose ID” to jump to the detail page of its conserved napRNA in Homo sapiens or Mus musculus.

⇒ Expression

• Step1: Users can click the Expression module on the homepage to search the expression levels of napRNA within the genome.
• Step2: In the "organism" section, select your target genome. In the "type" field, choose the napRNA of interest, then click "Submit". The expression levels of this napRNA in the genome will be displayed on a new page.
• Step3: Click "napRNA ID" to display the detail information about this napRNA. Click the "Plot" button to visualize this napRNA's expression levels across different cell types.

⇒ Predict

• Step1: Users can click the “Sequence” module on the homepage to query whether DNA sequences of interest contain napRNA.
• Step2: Select appropriate algorithms and seed values. Higher seed values yield more precise results, while lower values generate more matches (for details, refer to the blastn algorithm principle).
• Step3: Select a reference genome, enter your sequence, and click “Submit”. BLAST results will be displayed in a new page.

⇒ Gene

• Step1: Users can click the "Gene" module on the homepage to search napRNA by genes.
• Step2: Select a clade of interest, then choose your target organism, and finally select the gene before clicking "Submit". Information about the napRNA of host gene in organsim will be displayed on a new page.
• Step3: Click "Browser" to display the detail information about this napRNA. Click "Human ID" or “Mose ID” to jump to the detail page of its conserved napRNA in Homo sapiens or Mus musculus.

⇒ Relationships

• Step1: Users can explore napRNA's relationships with RNA modifications or RBP-binding events through the RNA Modifications module or RBP-binding Events module.
• Step2: Select your target organism, choose a napRNA type, and finally click "Submit".Click "napRNA ID" to display the detail information about this napRNA.
• Step3: When using the RNA Modifications module, users can click the value of the "modID" column to jump to rmbase to view the details of the RNA modification site. When using the RBP-binding Events module, users can click the value of the "Accession" column to jump the corresponding database to view the details of this RBP.

⇒ napRNA in Human Diseases

• Step1: Users can select the Diseases module within Relationships to view mutation information of napRNA across cancer types.
• Step2: Select your napRNA type and cancer type of interest, then click Submit. The mutation map of the napRNA in cancer will be displayed on a new page.